lysis buffer Search Results


tissue cell lysis buffer  (Gold Biotechnology Inc)
94
Gold Biotechnology Inc tissue cell lysis buffer
Tissue Cell Lysis Buffer, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tissue cell lysis buffer/product/Gold Biotechnology Inc
Average 94 stars, based on 1 article reviews
tissue cell lysis buffer - by Bioz Stars, 2026-04
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lysis buffer 17  (R&D Systems)
95
R&D Systems lysis buffer 17
Lysis Buffer 17, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
lysis buffer 17 - by Bioz Stars, 2026-04
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pbs  (R&D Systems)
95
R&D Systems pbs
Pbs, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbs/product/R&D Systems
Average 95 stars, based on 1 article reviews
pbs - by Bioz Stars, 2026-04
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red blood cell lysis  (R&D Systems)
94
R&D Systems red blood cell lysis
Red Blood Cell Lysis, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/red blood cell lysis/product/R&D Systems
Average 94 stars, based on 1 article reviews
red blood cell lysis - by Bioz Stars, 2026-04
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ice cold lysis buffer 6  (R&D Systems)
92
R&D Systems ice cold lysis buffer 6
Ice Cold Lysis Buffer 6, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ice cold lysis buffer 6/product/R&D Systems
Average 92 stars, based on 1 article reviews
ice cold lysis buffer 6 - by Bioz Stars, 2026-04
92/100 stars
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ripa buffer  (MedChemExpress)
97
MedChemExpress ripa buffer
Ripa Buffer, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 97 stars, based on 1 article reviews
ripa buffer - by Bioz Stars, 2026-04
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exosomes lysates  (R&D Systems)
91
R&D Systems exosomes lysates
Uptake of <t>exosomes</t> from high glucose-treated macrophages upregulates expression of inflammatory and fibrogenesis genes in fibroblasts. Characterization of exosomes from macrophage cell <t>line</t> <t>(RAW</t> 264.7 cells) by NanoSight dynamic light scattering analysis (A), transmission electron microscopy (B), and Western blotting and dot blot for exosome markers (C). Inset in panel B represents a closer view of the indicated region, scale bar, 100 μm. D, qRT-PCR data showing higher HuR mRNA levels in exosomes of high glucose-treated cells, compared with exosomes from normal glucose-treated cells (normalized to GAPDH, *P < .05). E, Western blot to show presence of HuR protein in macrophage exosome. F, Immunofluorescence staining showing uptake of PKH26-labeled macrophage exosomes (red) by fibroblasts (FB; NIH/3T3 fibroblast cell line; Flash Phalloidin green staining). DAPI to stain nuclei (blue); scale bar, 10 μm. G, qRT-PCR showing increase in mRNA expression of inflammatory and fibrogenesis-related genes in FB co-cultured with exosomes derived from high glucose-treated macrophages (Exo-HG), compared to FB treated with exosomes derived from normal glucose-treated macrophages (Exo-HG). Data normalized to β-actin expression. n = 3, *P < .01
Exosomes Lysates, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/exosomes lysates/product/R&D Systems
Average 91 stars, based on 1 article reviews
exosomes lysates - by Bioz Stars, 2026-04
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lysis buffer 16  (R&D Systems)
91
R&D Systems lysis buffer 16
Uptake of <t>exosomes</t> from high glucose-treated macrophages upregulates expression of inflammatory and fibrogenesis genes in fibroblasts. Characterization of exosomes from macrophage cell <t>line</t> <t>(RAW</t> 264.7 cells) by NanoSight dynamic light scattering analysis (A), transmission electron microscopy (B), and Western blotting and dot blot for exosome markers (C). Inset in panel B represents a closer view of the indicated region, scale bar, 100 μm. D, qRT-PCR data showing higher HuR mRNA levels in exosomes of high glucose-treated cells, compared with exosomes from normal glucose-treated cells (normalized to GAPDH, *P < .05). E, Western blot to show presence of HuR protein in macrophage exosome. F, Immunofluorescence staining showing uptake of PKH26-labeled macrophage exosomes (red) by fibroblasts (FB; NIH/3T3 fibroblast cell line; Flash Phalloidin green staining). DAPI to stain nuclei (blue); scale bar, 10 μm. G, qRT-PCR showing increase in mRNA expression of inflammatory and fibrogenesis-related genes in FB co-cultured with exosomes derived from high glucose-treated macrophages (Exo-HG), compared to FB treated with exosomes derived from normal glucose-treated macrophages (Exo-HG). Data normalized to β-actin expression. n = 3, *P < .01
Lysis Buffer 16, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lysis buffer 16/product/R&D Systems
Average 91 stars, based on 1 article reviews
lysis buffer 16 - by Bioz Stars, 2026-04
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ripa buffer  (Boston BioProducts)
98
Boston BioProducts ripa buffer
Uptake of <t>exosomes</t> from high glucose-treated macrophages upregulates expression of inflammatory and fibrogenesis genes in fibroblasts. Characterization of exosomes from macrophage cell <t>line</t> <t>(RAW</t> 264.7 cells) by NanoSight dynamic light scattering analysis (A), transmission electron microscopy (B), and Western blotting and dot blot for exosome markers (C). Inset in panel B represents a closer view of the indicated region, scale bar, 100 μm. D, qRT-PCR data showing higher HuR mRNA levels in exosomes of high glucose-treated cells, compared with exosomes from normal glucose-treated cells (normalized to GAPDH, *P < .05). E, Western blot to show presence of HuR protein in macrophage exosome. F, Immunofluorescence staining showing uptake of PKH26-labeled macrophage exosomes (red) by fibroblasts (FB; NIH/3T3 fibroblast cell line; Flash Phalloidin green staining). DAPI to stain nuclei (blue); scale bar, 10 μm. G, qRT-PCR showing increase in mRNA expression of inflammatory and fibrogenesis-related genes in FB co-cultured with exosomes derived from high glucose-treated macrophages (Exo-HG), compared to FB treated with exosomes derived from normal glucose-treated macrophages (Exo-HG). Data normalized to β-actin expression. n = 3, *P < .01
Ripa Buffer, supplied by Boston BioProducts, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ripa buffer/product/Boston BioProducts
Average 98 stars, based on 1 article reviews
ripa buffer - by Bioz Stars, 2026-04
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np 40 lysis buffer  (MedChemExpress)
95
MedChemExpress np 40 lysis buffer
Uptake of <t>exosomes</t> from high glucose-treated macrophages upregulates expression of inflammatory and fibrogenesis genes in fibroblasts. Characterization of exosomes from macrophage cell <t>line</t> <t>(RAW</t> 264.7 cells) by NanoSight dynamic light scattering analysis (A), transmission electron microscopy (B), and Western blotting and dot blot for exosome markers (C). Inset in panel B represents a closer view of the indicated region, scale bar, 100 μm. D, qRT-PCR data showing higher HuR mRNA levels in exosomes of high glucose-treated cells, compared with exosomes from normal glucose-treated cells (normalized to GAPDH, *P < .05). E, Western blot to show presence of HuR protein in macrophage exosome. F, Immunofluorescence staining showing uptake of PKH26-labeled macrophage exosomes (red) by fibroblasts (FB; NIH/3T3 fibroblast cell line; Flash Phalloidin green staining). DAPI to stain nuclei (blue); scale bar, 10 μm. G, qRT-PCR showing increase in mRNA expression of inflammatory and fibrogenesis-related genes in FB co-cultured with exosomes derived from high glucose-treated macrophages (Exo-HG), compared to FB treated with exosomes derived from normal glucose-treated macrophages (Exo-HG). Data normalized to β-actin expression. n = 3, *P < .01
Np 40 Lysis Buffer, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/np 40 lysis buffer/product/MedChemExpress
Average 95 stars, based on 1 article reviews
np 40 lysis buffer - by Bioz Stars, 2026-04
95/100 stars
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red blood cell lysis  (MedChemExpress)
93
MedChemExpress red blood cell lysis
Uptake of <t>exosomes</t> from high glucose-treated macrophages upregulates expression of inflammatory and fibrogenesis genes in fibroblasts. Characterization of exosomes from macrophage cell <t>line</t> <t>(RAW</t> 264.7 cells) by NanoSight dynamic light scattering analysis (A), transmission electron microscopy (B), and Western blotting and dot blot for exosome markers (C). Inset in panel B represents a closer view of the indicated region, scale bar, 100 μm. D, qRT-PCR data showing higher HuR mRNA levels in exosomes of high glucose-treated cells, compared with exosomes from normal glucose-treated cells (normalized to GAPDH, *P < .05). E, Western blot to show presence of HuR protein in macrophage exosome. F, Immunofluorescence staining showing uptake of PKH26-labeled macrophage exosomes (red) by fibroblasts (FB; NIH/3T3 fibroblast cell line; Flash Phalloidin green staining). DAPI to stain nuclei (blue); scale bar, 10 μm. G, qRT-PCR showing increase in mRNA expression of inflammatory and fibrogenesis-related genes in FB co-cultured with exosomes derived from high glucose-treated macrophages (Exo-HG), compared to FB treated with exosomes derived from normal glucose-treated macrophages (Exo-HG). Data normalized to β-actin expression. n = 3, *P < .01
Red Blood Cell Lysis, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/red blood cell lysis/product/MedChemExpress
Average 93 stars, based on 1 article reviews
red blood cell lysis - by Bioz Stars, 2026-04
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lysis buffer 15  (R&D Systems)
90
R&D Systems lysis buffer 15
Uptake of <t>exosomes</t> from high glucose-treated macrophages upregulates expression of inflammatory and fibrogenesis genes in fibroblasts. Characterization of exosomes from macrophage cell <t>line</t> <t>(RAW</t> 264.7 cells) by NanoSight dynamic light scattering analysis (A), transmission electron microscopy (B), and Western blotting and dot blot for exosome markers (C). Inset in panel B represents a closer view of the indicated region, scale bar, 100 μm. D, qRT-PCR data showing higher HuR mRNA levels in exosomes of high glucose-treated cells, compared with exosomes from normal glucose-treated cells (normalized to GAPDH, *P < .05). E, Western blot to show presence of HuR protein in macrophage exosome. F, Immunofluorescence staining showing uptake of PKH26-labeled macrophage exosomes (red) by fibroblasts (FB; NIH/3T3 fibroblast cell line; Flash Phalloidin green staining). DAPI to stain nuclei (blue); scale bar, 10 μm. G, qRT-PCR showing increase in mRNA expression of inflammatory and fibrogenesis-related genes in FB co-cultured with exosomes derived from high glucose-treated macrophages (Exo-HG), compared to FB treated with exosomes derived from normal glucose-treated macrophages (Exo-HG). Data normalized to β-actin expression. n = 3, *P < .01
Lysis Buffer 15, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lysis buffer 15/product/R&D Systems
Average 90 stars, based on 1 article reviews
lysis buffer 15 - by Bioz Stars, 2026-04
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Image Search Results


Uptake of exosomes from high glucose-treated macrophages upregulates expression of inflammatory and fibrogenesis genes in fibroblasts. Characterization of exosomes from macrophage cell line (RAW 264.7 cells) by NanoSight dynamic light scattering analysis (A), transmission electron microscopy (B), and Western blotting and dot blot for exosome markers (C). Inset in panel B represents a closer view of the indicated region, scale bar, 100 μm. D, qRT-PCR data showing higher HuR mRNA levels in exosomes of high glucose-treated cells, compared with exosomes from normal glucose-treated cells (normalized to GAPDH, *P < .05). E, Western blot to show presence of HuR protein in macrophage exosome. F, Immunofluorescence staining showing uptake of PKH26-labeled macrophage exosomes (red) by fibroblasts (FB; NIH/3T3 fibroblast cell line; Flash Phalloidin green staining). DAPI to stain nuclei (blue); scale bar, 10 μm. G, qRT-PCR showing increase in mRNA expression of inflammatory and fibrogenesis-related genes in FB co-cultured with exosomes derived from high glucose-treated macrophages (Exo-HG), compared to FB treated with exosomes derived from normal glucose-treated macrophages (Exo-HG). Data normalized to β-actin expression. n = 3, *P < .01

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Targeting exosome-associated human antigen R attenuates fibrosis and inflammation in diabetic heart

doi: 10.1096/fj.201901995R

Figure Lengend Snippet: Uptake of exosomes from high glucose-treated macrophages upregulates expression of inflammatory and fibrogenesis genes in fibroblasts. Characterization of exosomes from macrophage cell line (RAW 264.7 cells) by NanoSight dynamic light scattering analysis (A), transmission electron microscopy (B), and Western blotting and dot blot for exosome markers (C). Inset in panel B represents a closer view of the indicated region, scale bar, 100 μm. D, qRT-PCR data showing higher HuR mRNA levels in exosomes of high glucose-treated cells, compared with exosomes from normal glucose-treated cells (normalized to GAPDH, *P < .05). E, Western blot to show presence of HuR protein in macrophage exosome. F, Immunofluorescence staining showing uptake of PKH26-labeled macrophage exosomes (red) by fibroblasts (FB; NIH/3T3 fibroblast cell line; Flash Phalloidin green staining). DAPI to stain nuclei (blue); scale bar, 10 μm. G, qRT-PCR showing increase in mRNA expression of inflammatory and fibrogenesis-related genes in FB co-cultured with exosomes derived from high glucose-treated macrophages (Exo-HG), compared to FB treated with exosomes derived from normal glucose-treated macrophages (Exo-HG). Data normalized to β-actin expression. n = 3, *P < .01

Article Snippet: Protein extraction and Western blot analysis RAW 264.7 cells and exosomes lysates were prepared using cell lysis buffer 1 (Part No.: 890713, R & D Systems).

Techniques: Expressing, Transmission Assay, Electron Microscopy, Western Blot, Dot Blot, Quantitative RT-PCR, Immunofluorescence, Staining, Labeling, Cell Culture, Derivative Assay

Exosomes from high glucose-treated HuR-deficient macrophage abrogate inflammatory and fibrogenesis response in fibroblasts. Western blotting/densitometry analysis (A and B) and immunofluorescence staining (C) show efficient knockdown of HuR in mouse macrophage cell line (RAW 264.7) after 48 hours of lentiviral-based shRNA transduction. HuR KD, macrophage cells transduced with HuR-specific shRNA; WT, macrophage cells transduced with non-specific control shRNA. Scale bar, 20 μm. D, qRT-PCR analysis showing downregulation of mRNA related to inflammatory and/or fibrogenesis genes in fibroblasts (NIH3T3 cells) when co-cultured with exosomes from high glucose-treated HuR-deficient macrophage cells (HG-Exo-HGHuR KD). Data normalized to β-actin mRNA. (E and F) Western blot and densitometry analysis showing similar trend for MMP-9 and MMP13 proteins, data normalized to GAPDH. n = 3, *P < .01

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Targeting exosome-associated human antigen R attenuates fibrosis and inflammation in diabetic heart

doi: 10.1096/fj.201901995R

Figure Lengend Snippet: Exosomes from high glucose-treated HuR-deficient macrophage abrogate inflammatory and fibrogenesis response in fibroblasts. Western blotting/densitometry analysis (A and B) and immunofluorescence staining (C) show efficient knockdown of HuR in mouse macrophage cell line (RAW 264.7) after 48 hours of lentiviral-based shRNA transduction. HuR KD, macrophage cells transduced with HuR-specific shRNA; WT, macrophage cells transduced with non-specific control shRNA. Scale bar, 20 μm. D, qRT-PCR analysis showing downregulation of mRNA related to inflammatory and/or fibrogenesis genes in fibroblasts (NIH3T3 cells) when co-cultured with exosomes from high glucose-treated HuR-deficient macrophage cells (HG-Exo-HGHuR KD). Data normalized to β-actin mRNA. (E and F) Western blot and densitometry analysis showing similar trend for MMP-9 and MMP13 proteins, data normalized to GAPDH. n = 3, *P < .01

Article Snippet: Protein extraction and Western blot analysis RAW 264.7 cells and exosomes lysates were prepared using cell lysis buffer 1 (Part No.: 890713, R & D Systems).

Techniques: Western Blot, Immunofluorescence, Staining, Knockdown, shRNA, Transduction, Control, Quantitative RT-PCR, Cell Culture